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| 1. |  | MARTÍNEZ, M. H. P.; SILVA, D. G.; SILVA, J. S.; SILVA, A. E.; ALMEIDA, R. P. de. Repelência do pó de Rosmarinus officinalis L. sobre Tribolium castaneum (Coleoptera: Tenebrionidae) em sementes de amendoim. Cadernos de Agroecologia, Porto Alegre, v. 13, n. 1, mar. 2018 Edição dos anais do VI Congresso Latino-Americano (CLAA), X Congresso Brasileiro (CBA), V Seminário do DF e Entorno (SEMDF), 12-15 setembro de 2017, Brasília, DF, Brasil.| Biblioteca(s): Embrapa Algodão. |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
23/06/2017 |
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Data da última atualização: |
10/07/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CASTRO, V. L. S. S. de; CASTANHA, R. F.; VALLIM, J. H. |
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Afiliação: |
VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; RODRIGO FERNANDES CASTANHA, CNPMA; JOSE HENRIQUE VALLIM, CNPMA. |
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Título: |
Evaluation of NANoREG TiO2 toxicity in Caenorhabiditis elegans. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO ANUAL DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, 15., 2016, Campinas. [Abstracts...] Rio de Janeiro: Sociedade Brasileira de Pesquisas em Materiais, 2016. Resumo B4TJ. |
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Idioma: |
Inglês |
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Conteúdo: |
Caenorhabditis elegans is a suitable invertebrate alternative to vertebrate assays for ecotoxicological evaluations. NANoREG TiO2 2883578/ JRCNM01001a/990407 was tested by using Nanoreg SOPToxicity test with the nematode Caenorhabiditis elegans for the NANoREG core nanomaterials (Kleiven and Oughton, 2015). The strain of C.elegans used is the wild-type N2. The pre-culturing was performed in modified S-BASE media plus cholesterol, incubate at 20ºC, in darkness with shaking for 3-5 days. Age-synchronized worms were exposed to concentrations of TiO2 0.01 to 100 mg/L. Because test solution had no good dispersion, it was sonicated during 15 min, 400 W, 20 kHz before the test. Each test concentration was assayed in 4 replicates in 24-well tissues culture plates, with a total test volume of 1 mL/well containing EPA reconstituted water, Escherichia coli OP50, TiO2 and 5-20 juvenile L1 C.elegans. After 96 h, it was added 0.5 mL of Rose Bengal to each well and heated the multidishes in a drying oven for 10 min at 80 °C to kill the worms. To be able to estimate the growth (%) it was prepared a plate with 30 worms for the measure at time 0h. Four endpoints were determined: recovery, fertility, reproduction, and growth after 96 h of incubation. The recovery, fertility and reproduction were not affected by any of the concentrations. However, growth seems to be significantly reduced at concentration 100 mg/L (84%) in relation to control (115%). Responses in C. elegans depending not only on the concentration tested, but also on the endpoint. Growth seems to be a more sensitive endpoint to C. elegans than others evaluated. MenosCaenorhabditis elegans is a suitable invertebrate alternative to vertebrate assays for ecotoxicological evaluations. NANoREG TiO2 2883578/ JRCNM01001a/990407 was tested by using Nanoreg SOPToxicity test with the nematode Caenorhabiditis elegans for the NANoREG core nanomaterials (Kleiven and Oughton, 2015). The strain of C.elegans used is the wild-type N2. The pre-culturing was performed in modified S-BASE media plus cholesterol, incubate at 20ºC, in darkness with shaking for 3-5 days. Age-synchronized worms were exposed to concentrations of TiO2 0.01 to 100 mg/L. Because test solution had no good dispersion, it was sonicated during 15 min, 400 W, 20 kHz before the test. Each test concentration was assayed in 4 replicates in 24-well tissues culture plates, with a total test volume of 1 mL/well containing EPA reconstituted water, Escherichia coli OP50, TiO2 and 5-20 juvenile L1 C.elegans. After 96 h, it was added 0.5 mL of Rose Bengal to each well and heated the multidishes in a drying oven for 10 min at 80 °C to kill the worms. To be able to estimate the growth (%) it was prepared a plate with 30 worms for the measure at time 0h. Four endpoints were determined: recovery, fertility, reproduction, and growth after 96 h of incubation. The recovery, fertility and reproduction were not affected by any of the concentrations. However, growth seems to be significantly reduced at concentration 100 mg/L (84%) in relation to control (115%). Responses in C. elegans depending not only on... Mostrar Tudo |
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Thesagro: |
Toxicologia. |
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Thesaurus NAL: |
Caenorhabditis elegans. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161034/1/2016RA-093.pdf
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Marc: |
LEADER 02255nam a2200157 a 4500
001 2071457
005 2017-07-10
008 2016 bl uuuu u00u1 u #d
100 1 $aCASTRO, V. L. S. S. de
245 $aEvaluation of NANoREG TiO2 toxicity in Caenorhabiditis elegans.$h[electronic resource]
260 $aIn: ENCONTRO ANUAL DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, 15., 2016, Campinas. [Abstracts...] Rio de Janeiro: Sociedade Brasileira de Pesquisas em Materiais, 2016. Resumo B4TJ.$c2016
520 $aCaenorhabditis elegans is a suitable invertebrate alternative to vertebrate assays for ecotoxicological evaluations. NANoREG TiO2 2883578/ JRCNM01001a/990407 was tested by using Nanoreg SOPToxicity test with the nematode Caenorhabiditis elegans for the NANoREG core nanomaterials (Kleiven and Oughton, 2015). The strain of C.elegans used is the wild-type N2. The pre-culturing was performed in modified S-BASE media plus cholesterol, incubate at 20ºC, in darkness with shaking for 3-5 days. Age-synchronized worms were exposed to concentrations of TiO2 0.01 to 100 mg/L. Because test solution had no good dispersion, it was sonicated during 15 min, 400 W, 20 kHz before the test. Each test concentration was assayed in 4 replicates in 24-well tissues culture plates, with a total test volume of 1 mL/well containing EPA reconstituted water, Escherichia coli OP50, TiO2 and 5-20 juvenile L1 C.elegans. After 96 h, it was added 0.5 mL of Rose Bengal to each well and heated the multidishes in a drying oven for 10 min at 80 °C to kill the worms. To be able to estimate the growth (%) it was prepared a plate with 30 worms for the measure at time 0h. Four endpoints were determined: recovery, fertility, reproduction, and growth after 96 h of incubation. The recovery, fertility and reproduction were not affected by any of the concentrations. However, growth seems to be significantly reduced at concentration 100 mg/L (84%) in relation to control (115%). Responses in C. elegans depending not only on the concentration tested, but also on the endpoint. Growth seems to be a more sensitive endpoint to C. elegans than others evaluated.
650 $aCaenorhabditis elegans
650 $aToxicologia
700 1 $aCASTANHA, R. F.
700 1 $aVALLIM, J. H.
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Embrapa Meio Ambiente (CNPMA) |
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